Total erk antibody
WebProduct Specific Information. Description: The 5AD13MA monoclonal antibody recognizes human, mouse, and rat extracellular signal-regulated kinases 1 and 2 (also known as … WebJun 3, 2015 · I am using the following Antibodies from cell signaling - pAKT (9271) pERK(9101) and total ERK (9102). ... this is too large to be pAKT, pERK or total ERK.
Total erk antibody
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WebNormally in my laboratory, I use an antibody which detects Total Erk (both: the phosphorylated and the non-phosphorylated kinase) as a loading control for western blot, in apoptosis studies. WebApr 12, 2024 · HIGD2A promoted liver cancer cell growth by fueling mitochondrial ATP synthesis and activating the MAPK/ERK pathway, ... USA) for immunoblotting with the following primary antibodies: anti-HIGD2A antibody (ab150893, Abcam, USA), anti-Mfn1 antibody (13798-1-AP, ProteinTech ... Total RNA was extracted using the EZ-press RNA ...
WebSpecificity / Sensitivity. p44/42 MAP Kinase (L34F12) Mouse mAb detects endogenous levels of total p44/42 MAP kinase (Erk1/Erk2) protein. In some systems this antibody may … WebApr 5, 2024 · No significant differences of total ERK protein level were observed in U0126 conditions compared to non ... Membranes were stripped and reprobed with anti-pan-ERK antibody or with anti-α-tubulin ...
WebDetect and quantitate phosphorylated and total ERK1/2 simultaneously using Alpha SureFire,/i>® Ultra™ Multiplex. We are now two standalone organizations Life Sciences & Diagnostics and Analytical & Enterprise Solutions. Learn More.
WebFeb 8, 2024 · ERK 2 Antibody (C-14) is a rabbit polyclonal IgG; 200 µg/ml; or goat IgG; 200 µg/ml, sc-154-G; epitope mapping at the C-terminus of ERK 2 of rat origin; Discontinued …
WebThe assay will exclusively recognize both natural and recombinant ERK 1/2, when phosphorylated at Threonine 202 and Tyrosine 204. Principle of the method The phospho ERK1/2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. pete whisenant baseballWebMay 1, 2009 · For total ERK, membranes were stripped for 30 min in a solution of 62.5 mM Tris HCl, 2% SDS, and 100 mM β-mercaptoethanol, and reprobed using a mouse monoclonal antibody to total ERK (1:1000, #4696, Cell Signaling Technology, Beverly, MA) followed by an Alexa 680 conjugated goat anti-mouse secondary antibody (1 : 10 000, Molecular Probes). petewhittaker01WebSee: MAPK/ERK pathway. Transgenic gene knockout mice lacking MAPK1 have major defects in early development. Conditional deletion of Mapk1 in B cells showed a role for MAPK1 in T-cell-dependent antibody production. A dominant gain-of-function mutant of Mapk1 in transgenic mice showed a role for MAPK1 in T-cell development. pete whiskyWebSanta Cruz Biotechnology, Inc. offers a broad range of ERK antibodies. Select ERK antibodies from monoclonal antibodies listed below. View detailed ERK antibody specifications by linking to the specific product blocks. Select appropriate ERK antibodies for your research by isotype, epitope, applications and species reactivity. starting inboard motor out of waterWebHRP conjugated antibodies were used as the secondary antibodies. Data is expressed as percentage change in phosphorylated ERK1/2 (p-ERK1/2) over total ERK1/2 (t-ERK1/2) … starting in safe mode windows 11WebAug 1, 2003 · To determine whether primary Reed-Sternberg cells also aberrantly express p-ERK1/2, we studied sections of HD lymph nodes by immunohistochemical techniques using antibodies to total ERK and phospho-specific antibody to p-ERK1/2. Sections from normal tonsils (n = 4) were stained for total ERK and p-ERK and were used as controls. pete whitlockWebIn another experiment, cells were pretreated with 2 mg/mL integrin β1-neutralising antibody, total protein was extracted using lysis buffer to analyze FAK, p-FAK, Erk, pErk, Elk1, ... Erk, and Elk1 relative to total FAK, Erk, and Elk1 were gradually increased from 0.5 h to 12 h after IGFBP2 treatment, and then decreased from 12 h to 48 h ... pete whitlock marshall nc