Hbx and cccdna

Author: fvul

August undefined, 2024

WebHBx is responsible for the upregulation of E3 ubiquitin ligase MSL2, which contributes to HBV cccDNA activa-tion by inducing APOBEC3B degradation via ubiquity-lation of Lys320 site of APOBEC3B (Gao et al. 2024). Similarly, HBx/STAT3 signaling is stimulated by lncRNA HULC to facilitate production of miR-539 to downreg- WebFeb 29, 2024 · Our results highlight the ability of HBx to bind RNA to impact on the epigenetic control of both viral cccDNA and host genes and provide a new key to understand the role of DLEU2 and EZH2 overexpression in HBV-related HCCs and HBx contribution to hepatocytes transformation. Keywords: hepatocellular carcinoma, …

The progress of molecules and strategies for the treatment of HBV ...

WebHBx has been reported to affect HBV cccDNA treancription by interacting with cellular factors. To explore whether HBx could interact with YY1, and particularly, the spatial proximity between HBV DNA and 19q13.11 enhancer element, pCMV-HA-YY1 and pCMV-His-HBx plasmids were co-transfected in Huh7 cells and Co-IP assay was conducted. WebHBx is involved in viral replication, and it is required for HBV pregenomic RNA transcription. ( A ) HepG2 cells were transfected with monomeric linear full-length WT or HBx mutant … jefogarty pa.gov

Hepatitis B virus X protein counteracts high mobility group box 1

WebJan 1, 2024 · We then came to investigate whether HBx altered SMC3 accumulation on cccDNA. As expected, HBx deletion in MC-HBV (MC-HBVΔHBx) increased the abundance of SMC3 on cccDNA (Figure 10 A), and ectopic HBx expression significantly reduced the enrichment of SMC3 on cccDNA in HBV-infected HepG2 NTCP cells (Figure 10 B). WebSep 16, 2024 · The cccDNA genome is transcribed to different viral RNAs coding for HBx (0.7-kb RNA), three forms of HBsAg (2.4-kb RNA encoding the large and 2.1-kb RNA … WebFeb 27, 2024 · Sphondin treatment significantly reduced the recruitment of HBx to cccDNA, which subsequently led to inhibition of cccDNA transcription and HBsAg expression. The absence of HBx or R72A mutation potently abrogated the antiviral effect induced by sphondin in HBV-infected cells. jefo animal nutrition

HBx - an overview ScienceDirect Topics

WebSnippet: Transcription of hepatitis B virus (HBV) from the covalently closed circular DNA (cccDNA) template is essential for its replication. Suppressing the level and transcriptional activity of cccDNA might have anti-HBV effect. Although cellular transcription factors, such as CREB, which mediate HBV transcription, have been well described, transcriptional … WebSep 30, 2024 · 目前针对HBV cccDNA靶点治疗所开展的研究主要从两个方面进行，一方面是抑制HBV cccDNA的形成，如使用靶向病毒蛋白HBx和HBc负性调控cccDNA，以抑制其形成。但是该方法可能会靶向宿主蛋白，导致出现更多的毒副作用。 jefodWebNov 24, 2009 · HBx Is Required for HBV Pregenomic RNA Transcription. To investigate the mechanism involved in HBx-induced potentiation of HBV DNA replication, we measured the size of the cccDNA pool and the levels of pgRNA in HepG2 cells replicating either … We would like to show you a description here but the site won’t allow us. jefnje

"WebMar 1, 2024 · Thus, targeting HBx to silence cccDNA transcription could be an important curative strategy. We identified that the small molecule dicoumarol could block cccDNA … " - Hbx and cccdna

Hbx and cccdna

WebFeb 27, 2024 · Accumulating evidence have supported that HBx is associated with the cccDNA minichromosome and plays an important role in initiating and maintaining … WebHowever, HBx can relieve cccDNA transcriptional repression by restricting the expression of SIRT3 and blocking the recruitment of the proteins to cccDNA . As a major mitochondrial deacetylase, SIRT3 plays a crucial role in the regulation of ROS by targeting proteins involved in mitochondrial functions and antioxidant defenses . Ren et al ...

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WebMar 15, 2024 · A significant curative strategy, that is the functional silencing of cccDNA, might be achieved by targeting the viral protein HBx because of its crucial role in … WebApr 10, 2024 · HBx transactivates HAT1 and promotes histones acetylation on cccDNA to enhance cccDNA transcription, HBx also interacts with lncRNA DLEU2 to promote persistent cccDNA transcription. Therefore, identification of host factors targeted by HBx for promoting cccDNA transcription is of great importance, and is believed to be beneficial …

WebJul 7, 2024 · Under the idea that HBx already exists in the cell when cccDNA is generated, HBx could act as an adapter to connect cccDNA synthesis with DNA damage response pathways in these mechanisms. (26) The DNA polymerases H (POLH), K (POLK), and L (POLL) of the host have all been shown to have a significant contribution to the … WebApr 12, 2024 · HBx is a 154-amino acid (aa) protein that facilitates the efficient replication of HBV by stimulating HBV gene expression from the cccDNA template. However, the mechanism by which HBx...

WebFeb 29, 2024 · We explored, in relevant cellular models of HBV replication, the functional consequences of HBx interaction with DLEU2, a long non-coding RNA (lncRNA) … WebGiven the key role of HBx in cccDNA transcription, drugs targeting HBx may have the potential to inactivate cccDNA transcription. Nitazoxanide (NTZ), a thiazolide a broad …

WebSep 7, 2024 · IFN-α depresses the succinylation of histone H3K79 on HBV cccDNA minichromosome. (a) HepaRG cells were infected with HBV at an MOI of 600 vp/cell and treated with IFN-α for 3 days at the indicated dose. The levels of histone H3K27 acetylation and histone H3K79 succinylation were measured by Western blot analysis in the cells.

WebOct 27, 2024 · HBV encodes a regulatory protein, HBx, which activates transcription and replication of HBV genome organized as covalently closed circular (ccc) DNA minichromosome. Here we illustrate how HBx... je focusseWebAug 11, 2024 · In addition, HBx deficiency abrogated the inhibition of cccDNA transcription induced by rapamycin. Similar results were also confirmed in a recombinant cccDNA mouse model. In summary, we report a new small-molecule, rapamycin, which targets HBx to block HBV cccDNA transcription and inhibit HBV replication. lagu terang bulan negeri perakWebHigh levels of circulating antigens are thought to contribute to immune tolerance and HBV persistence. 4 In addition to the capsid protein (HBcAg) and the viral polymerase, HBV produces the regulatory X protein (HBx), … je focaliseWebPHB is characterized by the presence of viral DNA in the organism (including intrahepatic cccDNA and in-tegrated DNA). Possible virus persistence in the PHB patient’s hepatocytes potentiates the agent transmission risk via haemotransfusion, organ transplantation and haemodialysis. Occult HBV infection in PHB individuals can reactivate at jef odWebRecently, it was shown that HBx is essential for viral transcription from the cccDNA template, presumably by inducing epigenetic changes (eg, histone acetylation) (Fig. 18.11). It remains to be seen how HBx induces the epigenetic modification of the nuclear cccDNA template. Sign in to download full-size image Figure 18.11. jefo groupWebInterferon-α treatment and hepatitis B x protein (HBx) deletion were applied as two strategies for cccDNA repression. Chromatin immunoprecipitation and micrococcal … je fond je fondWebHepatitis B X protein (HBx) upregulated HSPA8 by coactivating the transcription factor heat shock factor 1 (HSF1) in cells. HSPA8 enhanced HBV replication by recruiting hepatitis B core protein (HBc) to the HBV covalently closed circular DNA (cccDNA) minichromosome, forming a positive feedback loop. lagu terapi otak